miR-4708의 multidrug resistance protein-1 발현 조절을 통한 paclitaxel 민감성 증가
miR-4708 Enhances Paclitaxel Sensitivity through Modulating Expression of Multidrug Resistance Protein-1
최장열, 유지원, 안상건,
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최장열 ( Choi Jang-Yeol ) - 조선대학교 치과대학 구강병리학교실
유지원 ( Ryu Ji-Won ) - 조선대학교 치의학전문대학원 구강내과학교실
안상건 ( Ahn Sang-Gun ) - 조선대학교 치과대학 구강병리학교실
KMID : 0829220170410020055
Abstract
Multidrug resistance (MDR) remains one of the most significant obstacles in various cancer treatment, and this process often involves dysregulation of the number of micro-RNAs. The aim of this study was to explore the role of miR-4708 on the regulation of MDR-1 expression and the regulation of multidrug resistance (MDR) to chemotherapeutic drugs. Luciferase reporter assays demonstrated that miR-4708 directly binds MDR-1 3’-UTR and down-regulated reporter luciferase activity. The mRNA and protein expression levels of MDR1 were significantly decreased following miR-4708 overexpression. Additionally, the accumulation of rhodamine-123 in paclitacel resistant FaDu cells following miR-4708 transfection was significantly increased compared with control, indicating that the efflux capacity was reduced. These results demonstrated that miR-4708 could be involved in the regulation of MDR via targeting MDR-1 and may provide a potential strategy for reversing drug resistance in oral cancer.
키워드
miR-4708; FaDu; MDR1; Paclitaxel
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